Nitrogen Removal Characteristics of a Cold-Tolerant Aerobic Denitrification Bacterium, Pseudomonas sp. 41

Nitrogen pollution of surface water is the main cause eutrophication, and considered a worldwide challenge in treatment. Currently, total nitrogen (TN) content effluent wastewater treatment plants (WWTPs) still high at low winter temperatures, mainly as result incomplete removal nitrate (NO3?-N). In this research, novel aerobic denitrifier identified Pseudomonas sp. 41 was isolated from municipal activated sludge; strain could rapidly degrade concentration NO3?-N temperature. Strain completely converted 100 mg/L 48 h 15 °C, maximum rate reached 4.0 mg/L/h. The functional genes napA, nirS, norB nosZ were successfully amplified, which provided theoretical support for denitrification capacity 41. particular, results experiments showed that perform under catalysis NAP. balance analysis revealed degraded through assimilation (52.35%) (44.02%), combined with gene amplification results, metabolism pathway proposed. Single-factor confirmed possessed best performance conditions sodium citrate carbon source, C/N ratio 10, pH 8, temperature 15–30 °C rotation speed 120 rpm. Meanwhile, bioaugmentation test manifested immobilized remarkably improved efficiency shortened reaction time synthetic wastewater.